Crews from County Durham & Darlington Fire and Rescue Service were on the scene of the blaze, behind Durham Fire Station on Lanchester Road, for around 19 hours.
The intial call came in on Tuesday, June 23 at around 10.20pm, with firefighters working through the night to bring the flames under control.
The fire was extinguished at 5pm the following day.
Durham Constabulary confirmed an arson investigation has been launched.
A force spokeswoman told the Echo: “We were called to a large barn fire on Lanchester Road, in Durham, at 10.30pm on Tuesday, June 23.
“Officers are treating the incident as arson and an investigation is ongoing into the circumstances surrounding the fire.
The scene of the fire on Wednesday, June 24.
“Anyone with any information that could help the investigation should call Durham Constabulary on 101, quoting incident number 486 of June 23.”
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