The Black Cats are ninth in the table following last weekend’s draw at Millwall, and were narrowly beaten by Premier League side Fulham on Wednesday.
Mowbray will have to weigh up whether to make changes to his side ahead of a congested fixture schedule.
Dennis Cirkin remains unavailable after suffering a concussion against Millwall last weekend, while Lynden Gooch is still expected to miss out with a hip flexor issue.
Ross Stewart, Corry Evans and Elliot Embleton remain out with long-term injuries.
Here's our predicted Sunderland line-up to face Reading:
1. GK: Anthony Patterson
After getting away with an early mistake when a Millwall goal was disallowed last weekend, the keeper made an excellent save to deny George Honeyman in the second half. Photo: Martin Swinney
2. RB: Trai Hume
The full-back has started Sunderland’s last eight games at right-back and has been in fine form. Photo: Frank Reid
3. CB: Daniel Ballard
Was excellent against his former club Millwall last week and produced another solid display against Fulham in the cup. Photo: Martin Swinney
4. CB: Danny Batth
Assisted Jewison Bennette’s late goal against Fulham during a brief spell up front. Has started the side’s last four games after recovering from a calf issue. Photo: Frank Reid