The video stars judges David Walliams, Simon Cowell, Amanda Holden and Alesha Dixon in a medieval skit ahead of the variety competition's return next month.
Ant McPartlin and Dec Donnelly do not appear at all despite presenting the ITV show since its launch in 2007.
However, the pair have been absent from trailers for the show before - the last being in 2014.
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The trailer was filmed in February and billed as celebrating the show's Royal Variety Performance prize - with Holden starring as Queen Elizabeth I and Dixon as Queen Victoria.
It comes a week after McPartlin, 42, was charged with drink-driving following his arrest on March 18, and returned to rehab.
He will appear in court on April 4.
The presenter stepped down from his TV commitments following the incident, with Donnelly set to present the final two Saturday Night Takeaway shows on his own.
The pair both took part in filming Britain's Got Talent audition rounds earlier this year but ITV are yet to confirm whether McPartlin will return for the whole of the forthcoming series.