Talented young striker Jordan Hugill has broken an ankle – and misses Seaham Red Star's home FA Carlsberg Vase tie against Stokesley tomorrow.
Hugill's impressive form had won him a trial with Sunderland – but that will have to go on the back burner.
The injury, and inevitable long lay-off, is a big blow to manager Andy Toman.
Secretary John Smith said: "He'll certainly be missed and it's sad for the lad who was due to go to the Stadium of Light this week for trials.
"Kevin Ball has been in touch and said that when Jordan is back on his feet he will get the club's physio to look after him.
"I think it's a great gesture, but it's the sort of treatment little clubs have come to expect from the Black Cats."
Star also miss Stephen Winspear, while Robert Betson needs a late fitness check.
Brian Linighan and Aaron Croft are back and Michael Patterson, signed from Ryhope CW, is set for his debut.
Smith added: "It's a big game for us and hopefully, with the right attitude, we can complete the double over Stokesley. We beat them 4-1 just a couple of weeks ago."
Chester-le-Street are in West Yorkshire to face Liversedge, with Josh Home-Jackson and Nick Kane, who both missed the last game, fit to return.
South Shields have an attractive home tie with Pontefract Colliers, but manager Gary Steadman misses suspended trio Stephen Ramsey, Craig Ellison or Hal Graham.
Johnny Wightman has completed his cricket commitments and goes straight into the squad along with Tony Coe.
"Confidence is good after winning four games on the bounce," said Mariners secretary Phil Reay.
"Last season, we struggled all the way but we are fifth-top and looking forward to giving our South Yorkshire visitors a good game."
Craig Robson, newly signed from Consett, will make his Ryton debut at home to Crook Town, while Ashington miss Mark O'Dea against Armthorpe
Horden CW aim to repeat the form which earned them a midweek FA Cup victory over Durham City when they go to Pickering Town.
Following two guelling matches against the UniBond Premier side, manager Simon Corbett delays announcing his squad.
In a limited First Division programme, FA Vase holders Whitley Bay, exempt until the second round proper, go to Consett in a crucial First
Whitley manager Ian Chandler misses Terry Burke, Mark Taylor and Chris Fawcett, while Lee Picton (groin) is doubtful.
Dunston UTS hope to complete a quick double over Esh Winning, following their midweek League Cup success.
Stephen Preen and Michael Dixon resume and manager Billy Irwin must choose between Liam Connell and Barry Poskett in goal.
Leaders Spennymoor host champions Newcastle Benfield, whose manager Paul Baker asaid: "Spennymoor have made great strides and will take some beating, but signing Shaun Bell, Chris Carr and Stuart Morris from Durham City has added quality to our squad. We might be the underdogs but we're looking forward to the game."
In Division Two, unbeaten leaders Sunderland RCA face revitalised Washington at Albany Park.
RCA conceded their first points of the season in midweek and will find the Newtowners fired up following their rise to seventh place.