TYNE and Wear Metro services between Pelaw and South Hylton will be suspended all day on Sunday, May 29, as Network Rail carry out work on the tracks.
The line between Pelaw and Hebburn will also be suspended until about 2pm.
There will be two bus replacement services in operation throughout the day, service 900 operating from Heworth to Hebburn until the line re-opens, and service 901 operating between Heworth and South Hylton.
Passengers should buy their Metro tickets on the bus replacement service.
Nexus, which owns and manages Metro, is spending £385million modernising stations, trains and infrastructure over the next 11 years.
Managing Director of Metro operator DB Regio Tyne and Wear (DBTW) Richard McClean, said: “This work is a vital part of the Metro: all change modernisation programme.
“Inconvenience to passengers will be kept to a minimum as essential engineering work is carried out on the network.
“We are sorry for any inconvenience caused by this work during this weekend.
“Normal service will resume at approximately 2pm on the line between Pelaw and Hebburn and at start of service on Monday, May 30, on the line between Pelaw and South Hylton. However, as it is a Bank Holiday, the service will operate to a Saturday timetable.”