National chain JD Wetherspoon has spent £1.2million revamping the old Blu Bambu nightclub, in Albion Place.
The new pub will be named The Cooper Rose, after a Victorian vaccination needle, and have a capacity of nearly 1,000 punters.
The development, which has created 50 jobs, will officially open on Tuesday.
However, some lucky customers – who have received a ticket from Wetherspoons – will be able to give Wearside’s latest watering hole a test run during the daytime today and over the weekend.
The company already runs The Lambton Worm pub, just down the road in Low Row, and The William Jameson, in Fawcett Street.
However, the new bar will be branded as a Lloyds No 1, and have music, DJs and a dance floor.
“It’s a Wetherspoons during the daytime,” said manager Nik Chapman.
“Then from about nine or 10pm we almost turn it into a nightclub.”
The new pub has a bar on each of its two floors, and will specialise in real ales.
The Cooper Rose will be open from 8am every day, until 1am on Wednesdays; 2am on Sundays, Tuesdays and Thursdays; 3am Mondays; and 4am Fridays and Saturdays. Children, accompanied by an adult, will be welcome until 9pm throughout the week, with last orders for food at 8pm. As a condition on the licence, both adult and child must order a meal.
The Cooper Rose building used to house Durham Book Centre, before it was turned into Beach nightclub in 2001.
It underwent a million pound refit in 2006 and was re-named Blu Bambu.
In August 2008, the two clubbers made front page news after a shocking mobile phone video appeared, which captured them cavorting naked on stage.
The five-minute film showed hundreds of drunken revellers watching, as the pair stripped off their clothes before simulating sex with each other.